General Course Information

OCB5990 - WS: Aquatic Flow Cytometry - Section 51 - Credits: 1

OCB5990 "Graduate Workshop in Aquatic Flow Cytometry": A practical introduction to theories and applications of flow cytometry in the analyses of aquatic microorganisms (bacteria, phytoplankton) and their physiology.

Time and Location: Weekly one afternoon. Course time will be arranged in consultation with course participants and announced here at a later time. Class room: instructor's research lab, AC-2 350 at Biscayne Bay Campus

Textbook: M.G. Omerod // Flow Cytometry, 2nd edition // Springer and Bios Scientific Publishers in association with the Royal Microscopic Society // (c) 1999 // ISBN 0-387-91587-7 // Price: paperback 109 pp, $39.95
Additional material maybe presented during the class.

Contact: Office: AC-1 Room 379; Phone: (305) 919 5882; e-mail: frank@jochem.net
Office hours: Wednesdays, 10:30 to 12:00 a.m. or by appointment (use preferably e-mail). Office hours are intended solely for discussion of academic material.

Significance of Course: The workshop will comprise weekly afternoon meetings with theory instruction, literature review and discussion, and practical experience on the FACSort flow cytometer in the instructor’s lab. Topics to be covered will be:

• Introduction: technical principles of flow cytometry and different sorts of cytometers
• Phytoplankton quantification: measurement of autofluorescent cells and the principles of fluorescence measurements
• Phytoplankton physiology: photoadaptation and the analysis of cellular pigment contents
• Phytoplankton cell cycles: DNA measurement in algae Kinetic measurements: assessing the metabolic rates of single cells
• Bacterioplankton: quantification and differentiation of planktonic bacteria by DNA staining
• Detecting apoptotic cell death: immunolabeling and flow cytometry
• Cell sorting: principles, differences and problems